nmt是基因功能的活体检测技术,已被103位诺贝尔奖得主所在单位,及北大、清华、中科院使用。nmt历*的今天
2014年12月16日,山东省农科院孔令安用nmt在frontiers in plant science上发表了标题为effects of high nh4+ on k+ uptake, culm mechanical strength and grain filling in wheat的研究成果。
期刊:frontiers in plant science
主题:高铵抑制小麦根部钾吸收
标题:effects of high nh4+on k+ uptake, culm mechanical strength and grain filling in wheat
影响因子:4.495
检测指标:k+流速
作者:山东省农科院孔令安
英文摘要
it is well established that a high external nh4+ concentration depresses many processes in plant development, but the underlying mechanisms are still not well understood. to determine whether the negative effects of high levels of nh4+ are related to competitive cation uptake, wheat was grown in a field with moderate (18 g n m−2) and high (30 g n m−2) supplies ofnh4+ in the presence or absence of additional k+ (6 g k2o m−2) to examine culm mechanical strength, the main components of the vascular bundle, nitrogen (n) remobilization and the grain-filling rate.
the results indicated that an excessive supply of nh4+ significantly decreased culm mechanical strength, the cellulose and lignin contents of vascular bundles, the n remobilization efficiency (nre) and the grain-filling rate compared with a moderate level of nh4+. the additional provision of k+ considerably alleviated these negative effects of high nh4+, resulting in a 19.41–26.95% increase in culm mechanical strength during grain filling and a 34.59% increase in the nre. an assay using the scanning ion-selective electrode technique (siet) showed that the net rate of transmembrane k+ influx decreased by 84.62%, and measurements using flame photometry demonstrated that the k+ content decreased by 36.13% in wheat plants subjected to high nh4+.
this study indicates that the effects of high nh4+ on culm mechanical strength, cellulose and lignin contents, the nre and the grain-filling rate are probably associated with inhibition of k+ uptake in wheat.
中文摘要(谷歌机翻)
*,外部nh4+ 的高浓度会抑制植物发育中的许多过程,但其潜在机理仍未得到很好的理解。为了确定高水平的nh4+的负面影响是否与竞争性阳离子摄入相关,将小麦种植在提供适量(18 g n m-2)和大量(30 g n m-2)nh4+的田地上在存在或不存在额外的k+(6 g k2o m-2)的情况下进行图4的测试,以检查茎秆机械强度,维管束的主要成分,氮(n)迁移和谷粒填充率。
结果表明,与中等水平的nh4+相比,过量供应nh4+会显着降低茎秆机械强度,维管束中纤维素和木质素的含量,氮的固定效率(nre)和籽粒填充率。额外提供的k+大大减轻了高nh4+的这些负面影响,导致籽粒灌浆期间茎秆机械强度提高了19.41–26.95%,nre增加了34.59%。使用扫描离子选择电极技术(siet)进行的分析表明,跨膜k +流入的净速率降低了84.62%,而使用火焰光度法的测量结果表明,在高nh4+的小麦植株中,k+含量降低了36.13%。。
这项研究表明,高nh4+对小麦茎秆机械强度,纤维素和木质素含量,nre和籽粒充实率的影响可能与抑制小麦吸收k+有关。
(b) the effects of 10 mm nh4+ on the kinetics of the net k+ fluxes in wheat root epidermal cells; each point is the mean from six individual plants. (c) each column presents
the mean values of the k+ fluxes measured in the root epidermal cells over a recording period of 30 min. nm: moderate nh4+,nh:10mm nh4+ treatment. the bars represent the standard deviation for six individual plants. the different letters indicated above each column refer to significant differences at the p < 0.05 level.
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