the detection of foodborne bacteria on beef: theapplication of the electronic nose
电子鼻用于牛肉食源性细菌检测
soad a abdallah, laila a al-shatti, ali f alhajraf, noura al-hammad, bashayer al-awadi
abstract
this study aims to investigate the application of a fast electronic nose system (cyranose 320) for detecting foodborne bacteria. the system proved to be very efficient in detecting microbes in beef and sausage samples. in the first part of the study, the total viable counts (tvc) from fresh and frozen beef samples were determined using the standard microbiological method and by the application of the electronic nose. the second part applied the electronic nose to beef before and after contamination with different bacterial pathogens separay: e. coli o157: h7, salmonellatyphimurium 857, staphylococcus aureusand pseudomonas aeruginosa 27853. the results revealed that the cyranose 320 can detect the tvc in different beef and sausage samples and quantify the volatile organic compounds produced at concentrations from 50 ppb to > 350 ppb. the concentrations of gases collected from the samples before and after separate contamination with these pathogenic bacteria were highly significantly correlated (p < 0.005). from this study one can conclude that the electronic nose system is a rapid way for detecting volatile organic compounds produced by foodborne bacteria that contaminate beef.
本研究旨在探讨快速电子鼻系统(cyranose320)在食源性细菌检测中的应用。该系统对牛肉和香肠样品中的微生物检测非常有效。在研究的部分,新鲜和冷冻牛肉样品的总活菌计数(tvc)是通过标准微生物法和电子鼻的应用来确定的。第二部分分别将电子鼻应用于牛肉被不同细菌性病原体污染前后:大肠杆菌o157:h7、沙门氏菌857、*29213、铜绿假单胞菌27853。结果表明,cyranose320可检测不同牛肉和香肠样品中的tvc,并可定量测定浓度为50 ppb至>350 ppb的挥发性有机化合物。与这些病原菌分离污染前后从样品中收集的气体浓度高度相关(p<0.005)。从这项研究可以得出结论,电子鼻系统是一种快速检测挥发性有机化合物产生的食源性细菌污染牛肉。
keywords:food, pathogens, rapid detection, electronic nose
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