中英文说明丨艾美捷 SiR-Actin试剂盒#CY-SC001
sir-actin has fluorescence, cell permeability, and high specificity for f-actin. sir-atin staining for endogenous f-actin does not require gene manipulation or overexpression. its emission in far-red light minimizes phototoxicity and the self fluorescence of the sample. sir-actin is compatible with gfp and/or m-cherry fluorescent proteins. it can be imaged using standard cy5 filters. sir-actin can be used for wide field of view, confocal, sim or sted imaging of living cells and tissues. the number of probes allows for 50 to 200 staining experiments* (* based on the following conditions: 0.5 1ml staining solution/0.5 1um probe concentration for staining experiments. the number of staining experiments can be further increased by reducing the volume or probe concentration).
sir action cell imaging
the imaging of sir action is best done using standard cy5 settings. after labeling, live cells can be immediately imaged without the need for washing steps. optionally, a simple washing step includes replacing the labeled solution with fresh culture medium without probes, which typically improves the signal-to-noise ratio. if time elapses for imaging, it is recommended to keep the probe concentration equal to or lower than 100 nm throughout the experiment to obtain a constant signal and avoid probe interference with actin dynamics (reducing cell proliferation). if cleaning cells
before imaging, the staining will last for several hours.
sir-actin具有荧光性、细胞通透性和对f-actin的高度特异性。sir-actin染色内源性的f-actin不需要基因操作或过表达。它在远红光中的发射最大限度地降低了光毒性和样品的自身荧光。sir-actin兼容gfp和/或m-cherry荧光蛋白质。它可以用标准的cy5滤镜进行成像。sir-actin可用于活细胞和组织的宽视场、共聚焦、sim或sted成像。探针数量允许50 - 200染色实验。*（*基于以下条件:0.5 - 1ml染色液/ 0.5 - 1um探针浓度的染色实验。染色实验的数量可以通过减少体积或探针浓度来进一步增加）。
艾美捷sir-actin试剂盒#cy-sc001物理特性：
abs 652 nm
em 674 nm
ε652 nm 1.0·105 mol-1·cm-1
mw 1241.6 g/mol
mf c71h88n8o10si
探针浓度（nm） 建议标记时间（h）*
> 1000 0.5 - 1
500 3 - 4
200 4 - 6
< 100 6 - 12
sir-actin试剂盒文献参考：
1. fluorogenic probes for live-cell imaging of the cytoskeleton, g. lukinavičius et al., nature methods, 11, 731–733 (2014)
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中英文说明丨艾美捷 SiR-Actin试剂盒#CY-SC001
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sir action cell imaging
the imaging of sir action is best done using standard cy5 settings. after labeling, live cells can be immediately imaged without the need for washing steps. optionally, a simple washing step includes replacing the labeled solution with fresh culture medium without probes, which typically improves the signal-to-noise ratio. if time elapses for imaging, it is recommended to keep the probe concentration equal to or lower than 100 nm throughout the experiment to obtain a constant signal and avoid probe interference with actin dynamics (reducing cell proliferation). if cleaning cells
before imaging, the staining will last for several hours.
sir-actin具有荧光性、细胞通透性和对f-actin的高度特异性。sir-actin染色内源性的f-actin不需要基因操作或过表达。它在远红光中的发射最大限度地降低了光毒性和样品的自身荧光。sir-actin兼容gfp和/或m-cherry荧光蛋白质。它可以用标准的cy5滤镜进行成像。sir-actin可用于活细胞和组织的宽视场、共聚焦、sim或sted成像。探针数量允许50 - 200染色实验。*（*基于以下条件:0.5 - 1ml染色液/ 0.5 - 1um探针浓度的染色实验。染色实验的数量可以通过减少体积或探针浓度来进一步增加）。
艾美捷sir-actin试剂盒#cy-sc001物理特性：
abs 652 nm
em 674 nm
ε652 nm 1.0·105 mol-1·cm-1
mw 1241.6 g/mol
mf c71h88n8o10si
探针浓度（nm） 建议标记时间（h）*
> 1000 0.5 - 1
500 3 - 4
200 4 - 6
< 100 6 - 12
sir-actin试剂盒文献参考：
1. fluorogenic probes for live-cell imaging of the cytoskeleton, g. lukinavičius et al., nature methods, 11, 731–733 (2014)
农药能治病也能致病
保温泵的特性及用途
用户选用拉力试验机的四大注意事项
AEG 调功器\2A400-350 HF RLP2
材料的体积和表面测试方法
中英文说明丨艾美捷 SiR-Actin试剂盒#CY-SC001
污水-地埋式污水处理设备
逆反射标志测量仪为道路交通安全保驾护航
地理标志产品 富平甜瓜 - 可溶性固形物测定、总酸测定
Kinovate连接载体-NITTOPHASE
拉曼光谱产生的原因及应用介绍
虫剂影响鸟类生态 仪器人浅谈生态保护
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2023年1-8月贵州省大数据领域项目投资和新型数字基础设施建设情况
紧固件螺纹摩擦系数测试仪哪家专业
矫直机种类、矫直溫度传动系统方法的规定
电加热夹层锅在使用一段时间之后应该如何维护与保养？
R412017959型德国AVENTICS换向阀主要性能
广东省工业设计协会关于发布《家用果蔬净化器》团体标准的公告
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