90plus纳米粒度仪应用案例-21
文献名:cytosolicdeliveryofmembrane-impermeablemoleculesindendriticcellsusingph-responsivecore−shellnanoparticles
作者:yuhuahu,tamaralitwin,arpunr.nagaraja,brandonkwong,joshuakatz,nickiwatson,darrellj.irvine
departmentofchemicalengineering,massachusettsinstituteoftechnology,77massachusettsavenue,cambridge,massachusetts02139,departmentofchemistry,massachusettsinstituteoftechnology,77massachusettsavenue,cambridge,massachusetts02139,departmentofmaterialsscienceandengineering,massachusettsinstituteoftechnology,77massachusettsavenue,cambridge,massachusetts02139,departmentofbiologicalengineering,massachusettsinstituteoftechnology,77massachusettsavenue,cambridge,massachusetts02139,andwhiteheadinstitute,9cambridgecenter,cambridge,massachusetts02142
摘要:polycationsthatabsorbprotonsinresponsetotheacidificationofendosomescantheoreticallydisruptthesevesiclesviathe“protonsponge”effect.toexploitthismechanism,wecreatednanoparticleswithasegregatedcore−shellstructureforefficient,noncytotoxicintracellulardrugdelivery.cross-linkedpolymernanoparticlesweresynthesizedwithaph-responsivecoreandhydrophilicchargedshelldesignedtodisruptendosomesandmediatedrug/cellbinding,respectively.bysequesteringtherelativelyhydrophobicph-responsivecorecomponentwithinamorehydrophilicph-insensitiveshell,nontoxicdeliveryofsmallmoleculesandproteinstothecytosolwasachievedindendriticcells,akeycelltypeofinterestinthecontextofvaccinesandimmunotherapy.
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90plus 纳米粒度仪应用案例-21
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