DBCO-Serinol Phosphoramidite说明书
dbco-serinol phosphoramidite说明书
产品描述
most popular non-nucleosidic phosphoramidites for modification and labeling are based on two structural types: 1,2-diols and 1,3-diols. products based on a 1,2-diol backbone were first described to allow amino-modification and biotin labeling. technically, the 1,2-diol backbone has some drawbacks relative to the 1,3-diol backbone. the 1,2-diol backbone can participate in a dephosphorylation reaction since the 1,2-diol can form a favored 5-membered cyclic phosphate intermediate. this reaction is competitive with simple hydrolysis of the protecting groups and leads to some loss of label. however, the degree of loss at the 3’ terminus can be limited by the removal of the cyanoethyl protecting group using dbu or diethylamine prior to the cleavage and deprotection steps. similarly, loss at the 5’ terminus can be eliminated by retaining the dmt group until the oligo is fully deprotected. fortunately, the elimination reaction is virtually non-existent in the 1,3-diol backbone since the cyclic intermediate would be a 6-membered ring which is not favored for a cyclic phosphate intermediate.
ivd customers have requested a new backbone based on a 1,3-diol that would overcome any technical or ip issues surrounding our current products. we now offer a line of products based on the serinol backbone, which have been developed in close collaboration between glen research and nelson biotechnologies. protected biotin serinol phosphoramidite and cpg are protected with a t-butylbenzoyl group on the biotin ring. this group is designed to stop any phosphoramidite reactions at this active position in biotin. this protection avoids branching when using nucleophilic activators like dci. the protecting group is easily removed during oligonucleotide cleavage and deprotection. the biotinlc versions are similarly protected and should be useful for the synthesis of highly sensitive biotinylated probes. 6-fluorescein serinol phosphoramidite and cpg are designed to prepare oligonucleotides containing one or several 6-fluorescein (6-fam) residues. amino-modifier serinol phosphoramidite and cpg are used to add amino groups into one or several positions in oligonucleotides. the amino group is protected with fmoc, which may be removed on the synthesis column prior to solid-phase conjugation to the amino groups, or which may be removed during deprotection for subsequent solution phase conjugation to the amino groups.
combining lipoic acid and our patented serinol backbone, we now offer dithiol serinol phosphoramidite and the related 3’-dithiol serinol cpg. this unique architecture moves the bulky dithiol away from the phosphate backbone, making it suitable for conjugation to gold surfaces. the long spacer arm of dithiol serinol also allows multiple consecutive incorporations of the modifier without the need for intermediate spacer phosphoramidite additions to achieve optimal stepwise coupling efficiency.
货号 品名 规格 品牌
78gg10003-0.25g dbco-serinol phosphoramidite 0.25g biohub
90度剥离强度试验机-数据可追溯
正确清洗二手不锈钢反应釜的重要性
B2级橡塑保温板厂家价格趋势
家庭自制油中极性标志物、酸价、水分的检测
施耐德电气推出Easergy MiCOM P5装置
DBCO-Serinol Phosphoramidite说明书
混凝土抗冲刷试验机混凝土冲刷试验机技术参数
COD测定仪的原理及参数配置
不锈钢板材自动化去毛刺 用哪种研磨刷比较好
英国ACE色谱柱的开发主要是为了解决色谱应用
河北专业做*空调管道铁皮保温施工队
行走减速机和卷扬减速机的区别
关于金相显微镜的使用教程
自动夹盖染料压盖机 6升染料压盖机
太原工地简易厕所的有效实际应用体现在哪些方面
小型数控车床的常见故障分析
恒奥德颗粒强度测定仪使用方法注意事项
50KN电子试验机报价
安晟集热式磁力搅拌器的三大优点
防撞软包材料选用什么材质好？防撞软包厂家
产品描述
most popular non-nucleosidic phosphoramidites for modification and labeling are based on two structural types: 1,2-diols and 1,3-diols. products based on a 1,2-diol backbone were first described to allow amino-modification and biotin labeling. technically, the 1,2-diol backbone has some drawbacks relative to the 1,3-diol backbone. the 1,2-diol backbone can participate in a dephosphorylation reaction since the 1,2-diol can form a favored 5-membered cyclic phosphate intermediate. this reaction is competitive with simple hydrolysis of the protecting groups and leads to some loss of label. however, the degree of loss at the 3’ terminus can be limited by the removal of the cyanoethyl protecting group using dbu or diethylamine prior to the cleavage and deprotection steps. similarly, loss at the 5’ terminus can be eliminated by retaining the dmt group until the oligo is fully deprotected. fortunately, the elimination reaction is virtually non-existent in the 1,3-diol backbone since the cyclic intermediate would be a 6-membered ring which is not favored for a cyclic phosphate intermediate.
ivd customers have requested a new backbone based on a 1,3-diol that would overcome any technical or ip issues surrounding our current products. we now offer a line of products based on the serinol backbone, which have been developed in close collaboration between glen research and nelson biotechnologies. protected biotin serinol phosphoramidite and cpg are protected with a t-butylbenzoyl group on the biotin ring. this group is designed to stop any phosphoramidite reactions at this active position in biotin. this protection avoids branching when using nucleophilic activators like dci. the protecting group is easily removed during oligonucleotide cleavage and deprotection. the biotinlc versions are similarly protected and should be useful for the synthesis of highly sensitive biotinylated probes. 6-fluorescein serinol phosphoramidite and cpg are designed to prepare oligonucleotides containing one or several 6-fluorescein (6-fam) residues. amino-modifier serinol phosphoramidite and cpg are used to add amino groups into one or several positions in oligonucleotides. the amino group is protected with fmoc, which may be removed on the synthesis column prior to solid-phase conjugation to the amino groups, or which may be removed during deprotection for subsequent solution phase conjugation to the amino groups.
combining lipoic acid and our patented serinol backbone, we now offer dithiol serinol phosphoramidite and the related 3’-dithiol serinol cpg. this unique architecture moves the bulky dithiol away from the phosphate backbone, making it suitable for conjugation to gold surfaces. the long spacer arm of dithiol serinol also allows multiple consecutive incorporations of the modifier without the need for intermediate spacer phosphoramidite additions to achieve optimal stepwise coupling efficiency.
货号 品名 规格 品牌
78gg10003-0.25g dbco-serinol phosphoramidite 0.25g biohub
90度剥离强度试验机-数据可追溯
正确清洗二手不锈钢反应釜的重要性
B2级橡塑保温板厂家价格趋势
家庭自制油中极性标志物、酸价、水分的检测
施耐德电气推出Easergy MiCOM P5装置
DBCO-Serinol Phosphoramidite说明书
混凝土抗冲刷试验机混凝土冲刷试验机技术参数
COD测定仪的原理及参数配置
不锈钢板材自动化去毛刺 用哪种研磨刷比较好
英国ACE色谱柱的开发主要是为了解决色谱应用
河北专业做*空调管道铁皮保温施工队
行走减速机和卷扬减速机的区别
关于金相显微镜的使用教程
自动夹盖染料压盖机 6升染料压盖机
太原工地简易厕所的有效实际应用体现在哪些方面
小型数控车床的常见故障分析
恒奥德颗粒强度测定仪使用方法注意事项
50KN电子试验机报价
安晟集热式磁力搅拌器的三大优点
防撞软包材料选用什么材质好？防撞软包厂家